MINI TOTAL RNA KIT (BLOOD & CULTURED CELLS)

Description

“Sample Size: 50-200µl whole human blood/up to 5×106 cultured mammalian cells/1×109 cultured bacterial cells

Expectant Yield: up to 5-30µg
Operation Time: 30min or less
Elution Volume: 50µl

Free sample available upon request

The Total RNA Mini Kit for blood and cultured cells is specially designed for purification of total RNA from fresh whole human blood and cultured cells. This method uses detergents and a chaotropic salt to lyse cells and inactivate RNase. The RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed following the wash step, the purified RNA is eluted by RNase-Free water. The entire procedure can be completed within 30 minutes. The purified RNA is ready for RT-PCR, Northern Blotting, Primer Extension, and cDNA library construction.

The quality of the Total RNA Mini Kit for Blood and Cultured Cells is tested on a lot-to-lot basis by isolating total RNA from blood and cultured cell samples. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.”

Additional Information

300 Preps