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Description
Strep B Carrot Broth™ is a one-step method for cultivation and identification of hemolytic strains of Group B Streptococcus.
This test is an improvement over conventional methods by:
- Decreasing turn around time
- Increasing sensitivity
- Lowering overall cost.
- Positive results for beta hemolytic strains can be observed as early as 6 hours after inoculation.
- If negative on Carrot Broth, non-hemolytic strains can be tested on Hardy’s GBS Detect plate (Cat. no. A300).
Procedures for processing clinical specimens for culture of group B Streptococcus (GBS)
“Remove swab(s) from transport medium. Inoculate swab(s) into a recommended selective broth medium, such as Todd-Hewitt broth supplemented with either gentamicin (8 µg/ml) and nalidixic acid (15 µg/ml) [TransVag broth], or with colistin (10 µg/ml) and nalidixic acid (15 µg/ml) [Lim broth]. TransVag broth may be supplemented with 5% defibrinated sheep blood to increase the recovery of GBS. As an alternative, swabs may be inoculated into selective enrichment broth that incorporates chromogenic pigments for the detection of beta-hemolytic GBS using color detection. Examples of appropriate commercially available options include StrepB carrot broth or Granada Biphasic broth.”
“Remove swab(s) from transport medium. Inoculate swab(s) into a recommended selective broth medium, such as Todd-Hewitt broth supplemented with either gentamicin (8 µg/ml) and nalidixic acid (15 µg/ml) [TransVag broth], or with colistin (10 µg/ml) and nalidixic acid (15 µg/ml) [Lim broth]. TransVag broth may be supplemented with 5% defibrinated sheep blood to increase the recovery of GBS. As an alternative, swabs may be inoculated into selective enrichment broth that incorporates chromogenic pigments for the detection of beta-hemolytic GBS using color detection. Examples of appropriate commercially available options include StrepB carrot broth or Granada Biphasic broth.”
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